Stable isotope tracing of trout hatchery carbon to sediments and foodwebs of limestone spring creeks.

Limestone springs support productive ecosystems and fisheries, yet aquaculture may modify or impair these ecosystems. We determined trout hatchery organic contribution to spring creek sediments and foodwebs with natural abundance stable isotope methods. Hatchery feed, waste, and trout were significantly enriched in delta(13)C relative to autotrophs and wild fish. Spring creek sediments were enriched in delta(13)C toward the hatchery endmember relative to reference streams without hatcheries and relative to a larger larger-order, spring-influenced stream. Contribution of hatchery C to spring creek sediments was greatest during March and associated with greatest sediment %C. Contribution of hatchery C to pollution-tolerant isopod diet was 39-51% in a stream receiving limestone spring water via hatchery effluent. Isopods of one spring creek also relied on hatchery-derived C within one month of hatchery closure. Four years later, less pollution pollution-tolerant amphipods dominated and consumed non-vascular over vascular autotrophs (86%). Isopods of a second spring creek with an active hatchery did not appear to be using hatchery matter directly, but were enriched in delta(34)S relative to a spring creek tributary with no hatchery influence. Isopods in both of these streams were relatively enriched in delta(15)N, indicating general nutrient enrichment from surrounding agricultural land use. The contribution of hatchery vs. wild fish in diet of herons and egrets was traced with delta(13)C of guano. These birds were strongly dependent on stocked trout in a spring creek with a recently closed state trout hatchery, and also near another large, state-run hatchery. Heron dependence on hatchery fish in the spring creek decreased with time since hatchery closure. Use of stable isotope natural abundance techniques in karst spring creeks can reveal stream impairment due to aquaculture, specific C sources to bio-indicating consumers, losses of farmed fish to predation, and potential exposure of higher order consumers to contaminants associated with aquaculture.